Covalently bound Bovine Serum Albumin (BSA) protein modified Hydrogenated Diamond Like Carbon (HDLC) surface as biosensor application

We have developed a biosensor based on BSA with the help of metal ions binding mechanism to detect and remove inorganic As (III), Cu (II), Pb (II) from water like fishing by hooking system. BSA was immobilized via DOPA onto the HDLC (named as BD-HDLC) surface having high loading capacity of proteins with no conformal change and having covalent interaction. These BD-HDLC samples, separately was immersed in the 25ml 1x10 -5 M metal ions solution in Tris buffers at pH 6.5, for 2 h at room temperature, under continuous slow stirring conditions using a magnetic stirrer. The metal ions treated BD-HDLC film was rinsed by ultrapure water and dried with nitrogen. We have studied that BD-HDLC surface shows high binding ligand activity to the potential binding site of Cu 2+ , Pb 2+ , and As 3+ . Additionally, BD-HDLC−Cu 2+ , BD-HDLC-Pb 2+ and BD-HDLC−As 3+ complexes can be used to study their individual interactions with each other. Thus, we demonstrate that excellent BD-HDLC can be used as a metal ion sensor at pH 6.5 upto 1X10 -5 M concentration of metal ion solution.